The group of 19 riders completed the challenge after two consecutive days of riding in aid of the Hornets’ self-financing Trust and Watford Mencap.
The team of intrepid cyclists set off early morning on Friday from the England capital and managed to reach the centre of Amsterdam on Saturday evening.
Season tickets holders and supporters of both charities, as well as representatives from a number of local companies partnered up for the cause including, Corona Energy, Mala Communications and Energie Fitness for Women.
Andrea Viglieno, trust business development executive and coordinator of the project, said: "I would like to thank Watford Mencap for the excellent partnership during these past months, Giant Radlett for their help and expertise and all the participants who took part to this rewarding challenge.
"Also a huge thank you to all who have donated."
Funds will be split 50/50 between the Trust and Watford Mencap and will be used to carry on the Trust and Mencap projects working alongside disabled, kids and young people in the local community
If anyone would like to take part next year, more details are available on the Community Trust website www.watfordfccsetrust.com.
The cyclists who took part were Andrea Viglieno, Liz Hizli, Andy Smith, Sam Breckon, John Breckon, Michael Moralee, Nigel Ellis, Steven Ellis, Paul Fegan, John Pilny, Michael Latta, Rishi Patel, Andrew Harrington, Luis Gisbert, Mark Racklyeft, Marcus Earnshaw, Rachel Schmitz, Graham Green and Lewis Cresswel.