A residents group opposed to the eruv in Bushey has started a petition in the hope that the county council will not grant the licence needed for it to go ahead.
Last August plans to erect a Jewish eruv, which will consist predominantly of 5.5 metre-high steel poles in 25 locations across Bushey, were approved by Hertsmere Borough Council.
The Bushey Residents Group formed last year in opposition of the eruv and a working group of seven to ten people meet regularly to plan the ongoing campaign.
The group do not believe they were properly consulted on the application.
The religious boundary around Bushey will turn the town into an area where strict Sabbath rules are relaxed for orthodox Jews, such as carrying or transporting items like wheelchairs, pushchairs and handkerchiefs.
To date 179 people have signed the petition on change.org. Although the planning permission has been granted, Hertfordshire County Council still need to grant a license for the application.