A quiz night held at The Three Crowns pub in Bushey has raised hundreds of pounds to help pay for a life changing operation for twins from Carpenders Park.
Rebecca and Jack Latham, from Hangar Ruding, were diagnosed with diplegic cerebral palsy just after their second birthday, and are unable to walk.
An operation not currently available on the NHS, called "selective dorsal rhizotomy" (SDR) treatment, could help both children walk, but will cost £25,000 to £30,000 for each child.
Mother Caroline Latham said: "They are bright, happy and fantastic little kids, when they play you wouldn't notice any difference, except when other children run off, Jack and Rebecca are left to crawl or grab a walking frame and struggle after them.
"It's heartbreaking to see."
The event held at the pub in High Road, Bushey Heath on Tuesday, October 2, collected £415.
But pub landlord Michael Duffy had a surprise for the family when he handed over the cheque last Tuesday, donating further cash to bring the total to up to £1,000.
Previously, The Watford Observer reported how the twins were bought secondhand trikes, which were modified by engineers at an Abbots Langley charity, with the costs, totalling £550, met by the Watford Lions.
The family has also raffled an Olympic Games Maker's t-shirt, signed players' cards from Manchester United and Arsenal, and a signed Bradley Wiggins cycling jersey to raise cash.