Watford Ladies Under-11s lost ground in their battle against relegation after being beaten 3-1 by Bury Rangers last Saturday.
Bury took a 2-0 lead before Kayra Karakus scored from distance for the Hornets.
Watford couldn't build on that goal and Bury bagged a third.
Watford Ladies Reserves were beaten in disappointing fashion, losing 5-0 to Millwall Lioness' on Sunday prior to Wednesday's Women's Super League opener between the two.
In the John Greenacre Memorial Trophy on Sunday, Watford Ladies Development side progressed to the semi-finals with a 2-0 win at Cobham.
The Under-16 Hornets beat Harvesters 2-1 on Saturday, the goals coming from Anna Re and Emily Jokiel.
Watford Ladies Under-14 Hornets thumped St Albans City South 9-0 on the same day, with Tabatha Cown, Ciara John, Danielle Maloney, Rebecca McShane and Olivia Syson adding to braces from Theodora Nicholson and Eleanor Lord.
Watford Under-14 Ladies Wasps went head-to-head with the Under-14 Ladies Herts, winning 2-0 thanks to a Sophia Citro double.
Finally, the Under-10 Ladies won 5-0 at Hitchin Belles. Hannah Perkins, Emelia Barton, Jessica Cripps all scored, with Erin O'Connor grabbing two.